Berkeley Lights launches cell line development 2.0 workflow to find top clones for complex antibody therapeutics

: The Opto Cell Line Development 2.0 workflow, designed for the Beacon® optofluidic instrument, generates in just days the best production cell lines for complex antibody therapeutics with more than 99% monoclonality assurance

Berkeley Lights, Cell line development, 2.0 workflow, Top clones, Complex antibody therapeutics, SpotLight™, CLD

Berkeley Lights, a leader in cell selection, launched a new workflow for the Beacon instrument: the Opto Cell Line Development 2.0 (Opto CLD 2.0). While it takes traditional well plate workflows eight to twelve weeks to find clones producing traditional antibodies, Opto CLD 2.0 enables selection of top clones secreting complex antibody molecules with superior clonality assurance in less than one week.

The new Opto CLD 2.0 workflow enables measurements of antibody production across thousands of clones using Berkeley Lights' SpotLight™ assays.  It targets different regions of antibody molecules, extending the range of complex antibody molecules for which top clones can be selected. Screens can also be directed at clones with specific phenotypes, such as size, viability, or expression of cell-surface markers.

"The last decade has seen an explosion in more complex antibody molecules that have unique therapeutic functions, such as Fc-engineered mAbs, bispecifics and multispecifics," said John Proctor, Ph.D., Senior Vice President of Marketing at Berkeley Lights. "By developing new assays that target different regions of antibody molecules, Berkeley Lights Opto CLD 2.0 workflow empowers the selection of top clones secreting both traditional and non-traditional antibody molecules."

Opto CLD 2.0 builds on existing CLD capabilities on the Beacon instrument to screen and select clones with 1.5–3x higher titers than clones selected by traditional clone-picking technologies, which by comparison require a slow, laborious, and inefficient process. Cells can be visually tracked from the moment they are cloned into NanoPenTM chambers through multiple days of on-chip culture, assay measurements, and clone recovery. Top clones can be recovered with more than 99% monoclonality assurance and a superior data package that includes individual visual records of all clones.